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dc.contributor.authorDöğen, Aylin
dc.contributor.authorMetin, Banu
dc.contributor.authorIlkit, Macit
dc.contributor.authorde Hoog, G. S.
dc.contributor.authorHeitman, Joseph
dc.date.accessioned2019-08-31T12:10:23Z
dc.date.accessioned2019-08-13T09:37:43Z
dc.date.available2019-08-31T12:10:23Z
dc.date.available2019-08-13T09:37:43Z
dc.date.issued2017en_US
dc.identifier.issn0933-7407
dc.identifier.issn1439-0507
dc.identifier.urihttps://hdl.handle.net/20.500.12436/1008
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0182653
dc.descriptionWOS: 000408951400024en_US
dc.description.abstractReference isolates of Candida parapsilosis (n = 8), Candida metapsilosis (n = 6), Candida orthopsilosis (n = 7), and Lodderomyces elongisporus (n = 11) were analyzed to gain insight into their pathobiology and virulence mechanisms. Initial evaluation using BBL Chromagar Candida medium misidentified L. elongisporus isolates as C. albicans. Polymerase chain reaction analysis of isolate MTL idiomorphs revealed that all C. parapsilosis isolates were MTLa homozygous and no MTL α1, α2, a1, or a2 gene was detected in L. elongisporus isolates. For C. orthopsilosis, two isolates were MTLa homozygous and five were MTL-heterozygous. Similarly, one C. metapsilosis isolate was MTLα homozygous whereas five were MTL-heterozygous. Isolate phenotypic switching analysis revealed potential phenotypic switching in the MTLα homozygous C. metapsilosis isolate, resulting in concomitant elongated cell formation. Minimum inhibitory concentrations of fluconazole (FLC) and FK506, alone or in combination, were determined by checkerboard assay, with data analyzed using the fractional inhibitory concentration index model. Synergistic or additive effects of these compounds were commonly observed in C. parapsilosis and L. elongisporus isolates. No killer activity was observed in the studied isolates, as determined phenotypically. No significant difference in virulence was seen for the four species in a Galleria mellonella model (P > 0.05). In conclusion, our results demonstrated phenotypic switching of C. metapsilosis CBS 2315 and that FLC and FK506 represent a promising drug combination against C. parapsilosis and L. elongisporus. The findings of the present study contribute to our understanding of the biology, diagnosis, and new possible treatments of the C. parapsilosis species group and L. elongisporus.
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.ispartofMycosesen_US
dc.identifier.doi10.1371/journal.pone.0182653
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleMTL genotypes, phenotypic switching, and susceptibility profiles of Candida parapsilosis species group compared to Lodderomyces elongisporusen_US
dc.typeconferenceObjecten_US
dc.departmentMühendislik ve Doğa Bilimleri Fakültesien_US
dc.identifier.volume60en_US
dc.identifier.startpage13en_US
dc.identifier.endpage13en_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.institutionauthorMetin, Banu
dc.department-temp[Dogen, A.] Mersin Univ, Dept Pharmaceut Microbiol, Fac Pharm, Mersin, Turkey; [Metin, B.] Istanbul Sabahattin Zaim Univ, Dept Food Engn, Fac Engn & Nat Sci, Istanbul, Turkey; [Ilkit, M.] Univ Cukurova, Div Mycol, Dept Microbiol, Fac Med, Adana, Turkey; [de Hoog, G. S.] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands; [de Hoog, G. S.] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands; [Heitman, J.] Duke Univ, Sch Med, Dept Mol Genet & Microbiol, Durham, NC USAen_US


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