Quechers method for simultaneous determination of veterinary drugs and pesticides anaylsis in milk by LC-MS/MS

Abstract

Veterinary drugs are used for disease treatment and prevention and also for improvement of growth and feeding efficiency. Incorrect use of these drugs may cause accumulation of such drugs in the animal body and intake by human. Therefore they should be checked to protect the agricultural environment and food industry Since there are many veterinary drugs to be monitored, development of methods enabling a wide number of drugs with multiple classes by a single analysis procedure is quite important. In this study, we prepared milk samples by using a procedure based on a simple liquid–liquid extraction. This method utilized a simple and quick sample preparation procedure using a single extraction step. (A. Kaufmann 2014 and R. Souza 2016) Additionally, reduced amount of chemicals and steps in sample preparation phase together with the avoidance of a sample clean-up step simplify the sample pretreatment and reduce the total laboratory costs. The proposed method reduced costs of analysis and improved the simplicity and provided high recoveries of compounds of various polarities. Using the method, milk samples were analysed for both veterinary drugs and pesticide residues simultaneously (Y. Zhang 2015 and H. G. Mol 2008) using liquid chromatography tandem mass spectrometry (LC–MS/MS). MS/MS PARAMETRELERİ LC Conclusions A multi-class multi-residue procedure with LC-MS/MS detection has been developed and validated to determine and quantify veterinary and pesticide residues in milk. A simple sample preparation method was used involved liquid extraction salting out procedures in ethyl acetate system, without clean-up steps, and shortening the sample preparation time. Validation of the method was performed according to Commission Decision 2002/657/EC. The method was characterized by good results in terms of recovery, reproducibility and repeatability allowing the detection of veterinary drug and pesticide residues below the recommended analytical level. Based on these results, LC-MS/MS method with ethyl acetate extraction showed the suitability for sensitive quantification of veterinary and pesticide residues in milk samples for food safety applications. The validated method was applied on 220 real commercial samples.class, multi-residue analysis of trace veterinary drugs in milk by rapid screening and quantification using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry, "